                               SEQUENCE LISTING

<110> DANISCO US INC., GENENCOR DIVISION 
 
<120> VARIANTS OF BACILLUS LICHENIFORMIS ALPHA-AMYLASE WITH INCREASED 
      THERMOSTABILITY AND/OR DECREASED CALCIUM DEPENDENCE

<130> 48452-0026-00-WO

<140>
<141>

<150> 60/985,619
<151> 2007-11-05

<160> 6     

<170> PatentIn version 3.5

<210> 1
<211> 1536
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      polynucleotide"

<400> 1
atgaaacaac aaaaacggct ttacgcccga ttgctgacgc tgttatttgc gctcatcttc     60

ttgctgcctc attctgcagc ttcagcagca aatcttaatg ggacgctgat gcagtatttt    120

gaatggtaca tgcccaatga cggccaacat tggaagcgtt tgcaaaacga ctcggcatat    180

ttggctgaac acggtattac tgccgtctgg attcccccgg catataaggg aacgagccaa    240

gcggatgtgg gctacggtgc ttacgacctt tatgatttag gggagtttca tcaaaaaggg    300

acggttcgga caaagtacgg cacaaaagga gagctgcaat ctgcgatcaa aagtcttcat    360

tcccgcgaca ttaacgttta cggggatgtg gtcatcaacc acaaaggcgg cgctgatgcg    420

accgaagatg taaccgcggt tgaagtcgat cccgctgacc gcaaccgcgt aatttcagga    480

gaacacctaa ttaaagcctg gacacatttt cattttccgg ggcgcggcag cacatacagc    540

gattttaaat ggcattggta ccattttgac ggaaccgatt gggacgagtc ccgaaagctg    600

aaccgcatct ataagtttca aggaaaggct tgggattggg aagtttccaa tgaaaacggc    660

aactatgatt atttgatgta tgccgacatc gattatgacc atcctgatgt cgcagcagaa    720

attaagagat ggggcacttg gtatgccaat gaactgcaat tggacggttt ccgtcttgat    780

gctgtcaaac acattaaatt tcaatttttg cgggattggg ttaatcatgt cagggaaaaa    840

acggggaagg aaatgtttac ggtagctgaa tattggcaga atgacttggg cgcgctggaa    900

aactatttga acaaaacaaa ttttaatcat tcagtgtttg acgtgccgct tcattatcag    960

ttccatgctg catcgacaca gggaggcggc tatgatatga ggaaattgct gaacggtacg   1020

gtcgtttcca agcatccgtt gaaatcggtt acatttgtcg ataaccatga tacacagccg   1080

gggcaatcgc ttgagtcgac tgtccaaaca tggtttaagc cgcttgctta cgcttttatt   1140

ctcacaaggg aatctggata ccctcaggtt ttctacgggg atatgtacgg gacgaaagga   1200

gactcccagc gcgaaattcc tgccttgaaa cacaaaattg aaccgatctt aaaagcgaga   1260

aaacagtatg cgtacggagc acagcatgat tatttcgacc accatgacat tgtcggctgg   1320

acaagggaag gcgacagctc ggttgcaaat tcaggtttgg cggcattaat aacagacgga   1380

cccggtgggg caaagcgaat gtatgtcggc cggcaaaacg ccggtgagac atggcatgac   1440

attaccggaa accgttcgga gccggttgtc atcaattcgg aaggctgggg agagtttcac   1500

gtaaacggcg ggtcggtttc aatttatgtt caaaga                             1536


<210> 2
<211> 483
<212> PRT
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      polypeptide"

<400> 2
Ala Asn Leu Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Met Pro 
1               5                   10                  15      


Asn Asp Gly Gln His Trp Lys Arg Leu Gln Asn Asp Ser Ala Tyr Leu 
            20                  25                  30          


Ala Glu His Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Tyr Lys Gly 
        35                  40                  45              


Thr Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu 
    50                  55                  60                  


Gly Glu Phe His Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 
65                  70                  75                  80  


Gly Glu Leu Gln Ser Ala Ile Lys Ser Leu His Ser Arg Asp Ile Asn 
                85                  90                  95      


Val Tyr Gly Asp Val Val Ile Asn His Lys Gly Gly Ala Asp Ala Thr 
            100                 105                 110         


Glu Asp Val Thr Ala Val Glu Val Asp Pro Ala Asp Arg Asn Arg Val 
        115                 120                 125             


Ile Ser Gly Glu His Leu Ile Lys Ala Trp Thr His Phe His Phe Pro 
    130                 135                 140                 


Gly Arg Gly Ser Thr Tyr Ser Asp Phe Lys Trp His Trp Tyr His Phe 
145                 150                 155                 160 


Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile Tyr Lys 
                165                 170                 175     


Phe Gln Gly Lys Ala Trp Asp Trp Glu Val Ser Asn Glu Asn Gly Asn 
            180                 185                 190         


Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr Asp His Pro Asp Val 
        195                 200                 205             


Ala Ala Glu Ile Lys Arg Trp Gly Thr Trp Tyr Ala Asn Glu Leu Gln 
    210                 215                 220                 


Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys Phe Gln Phe 
225                 230                 235                 240 


Leu Arg Asp Trp Val Asn His Val Arg Glu Lys Thr Gly Lys Glu Met 
                245                 250                 255     


Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu Glu Asn 
            260                 265                 270         


Tyr Leu Asn Lys Thr Asn Phe Asn His Ser Val Phe Asp Val Pro Leu 
        275                 280                 285             


His Tyr Gln Phe His Ala Ala Ser Thr Gln Gly Gly Gly Tyr Asp Met 
    290                 295                 300                 


Arg Lys Leu Leu Asn Gly Thr Val Val Ser Lys His Pro Leu Lys Ser 
305                 310                 315                 320 


Val Thr Phe Val Asp Asn His Asp Thr Gln Pro Gly Gln Ser Leu Glu 
                325                 330                 335     


Ser Thr Val Gln Thr Trp Phe Lys Pro Leu Ala Tyr Ala Phe Ile Leu 
            340                 345                 350         


Thr Arg Glu Ser Gly Tyr Pro Gln Val Phe Tyr Gly Asp Met Tyr Gly 
        355                 360                 365             


Thr Lys Gly Asp Ser Gln Arg Glu Ile Pro Ala Leu Lys His Lys Ile 
    370                 375                 380                 


Glu Pro Ile Leu Lys Ala Arg Lys Gln Tyr Ala Tyr Gly Ala Gln His 
385                 390                 395                 400 


Asp Tyr Phe Asp His His Asp Ile Val Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     


Ser Ser Val Ala Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ala Lys Arg Met Tyr Val Gly Arg Gln Asn Ala Gly Glu Thr 
        435                 440                 445             


Trp His Asp Ile Thr Gly Asn Arg Ser Glu Pro Val Val Ile Asn Ser 
    450                 455                 460                 


Glu Gly Trp Gly Glu Phe His Val Asn Gly Gly Ser Val Ser Ile Tyr 
465                 470                 475                 480 


Val Gln Arg 
            


<210> 3
<211> 1536
<212> DNA
<213> Bacillus licheniformis

<400> 3
atgaaacaac aaaaacggct ttacgcccga ttgctgacgc tgttatttgc gctcatcttc     60

ttgctgcctc attctgcagc ttcagcagca aatcttaatg ggacgctgat gcagtatttt    120

gaatggtaca tgcccaatga cggccaacat tggaagcgtt tgcaaaacga ctcggcatat    180

ttggctgaac acggtattac tgccgtctgg attcccccgg catataaggg aacgagccaa    240

gcggatgtgg gctacggtgc ttacgacctt tatgatttag gggagtttca tcaaaaaggg    300

acggttcgga caaagtacgg cacaaaagga gagctgcaat ctgcgatcaa aagtcttcat    360

tcccgcgaca ttaacgttta cggggatgtg gtcatcaacc acaaaggcgg cgctgatgcg    420

accgaagatg taaccgcggt tgaagtcgat cccgctgacc gcaaccgcgt aatttcagga    480

gaacacctaa ttaaagcctg gacacatttt cattttccgg ggcgcggcag cacatacagc    540

gattttaaat ggcattggta ccattttgac ggaaccgatt gggacgagtc ccgaaagctg    600

aaccgcatct ataagtttca aggaaaggct tgggattggg aagtttccaa tgaaaacggc    660

aactatgatt atttgatgta tgccgacatc gattatgacc atcctgatgt cgcagcagaa    720

attaagagat ggggcacttg gtatgccaat gaactgcaat tggacggttt ccgtcttgat    780

gctgtcaaac acattaaatt ttcttttttg cgggattggg ttaatcatgt cagggaaaaa    840

acggggaagg aaatgtttac ggtagctgaa tattggcaga atgacttggg cgcgctggaa    900

aactatttga acaaaacaaa ttttaatcat tcagtgtttg acgtgccgct tcattatcag    960

ttccatgctg catcgacaca gggaggcggc tatgatatga ggaaattgct gaacggtacg   1020

gtcgtttcca agcatccgtt gaaatcggtt acatttgtcg ataaccatga tacacagccg   1080

gggcaatcgc ttgagtcgac tgtccaaaca tggtttaagc cgcttgctta cgcttttatt   1140

ctcacaaggg aatctggata ccctcaggtt ttctacgggg atatgtacgg gacgaaagga   1200

gactcccagc gcgaaattcc tgccttgaaa cacaaaattg aaccgatctt aaaagcgaga   1260

aaacagtatg cgtacggagc acagcatgat tatttcgacc accatgacat tgtcggctgg   1320

acaagggaag gcgacagctc ggttgcaaat tcaggtttgg cggcattaat aacagacgga   1380

cccggtgggg caaagcgaat gtatgtcggc cggcaaaacg ccggtgagac atggcatgac   1440

attaccggaa accgttcgga gccggttgtc atcaattcgg aaggctgggg agagtttcac   1500

gtaaacggcg ggtcggtttc aatttatgtt caaaga                             1536


<210> 4
<211> 483
<212> PRT
<213> Bacillus licheniformis

<400> 4
Ala Asn Leu Asn Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Met Pro 
1               5                   10                  15      


Asn Asp Gly Gln His Trp Lys Arg Leu Gln Asn Asp Ser Ala Tyr Leu 
            20                  25                  30          


Ala Glu His Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Tyr Lys Gly 
        35                  40                  45              


Thr Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu 
    50                  55                  60                  


Gly Glu Phe His Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 
65                  70                  75                  80  


Gly Glu Leu Gln Ser Ala Ile Lys Ser Leu His Ser Arg Asp Ile Asn 
                85                  90                  95      


Val Tyr Gly Asp Val Val Ile Asn His Lys Gly Gly Ala Asp Ala Thr 
            100                 105                 110         


Glu Asp Val Thr Ala Val Glu Val Asp Pro Ala Asp Arg Asn Arg Val 
        115                 120                 125             


Ile Ser Gly Glu His Leu Ile Lys Ala Trp Thr His Phe His Phe Pro 
    130                 135                 140                 


Gly Arg Gly Ser Thr Tyr Ser Asp Phe Lys Trp His Trp Tyr His Phe 
145                 150                 155                 160 


Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile Tyr Lys 
                165                 170                 175     


Phe Gln Gly Lys Ala Trp Asp Trp Glu Val Ser Asn Glu Asn Gly Asn 
            180                 185                 190         


Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr Asp His Pro Asp Val 
        195                 200                 205             


Ala Ala Glu Ile Lys Arg Trp Gly Thr Trp Tyr Ala Asn Glu Leu Gln 
    210                 215                 220                 


Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys Phe Ser Phe 
225                 230                 235                 240 


Leu Arg Asp Trp Val Asn His Val Arg Glu Lys Thr Gly Lys Glu Met 
                245                 250                 255     


Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu Glu Asn 
            260                 265                 270         


Tyr Leu Asn Lys Thr Asn Phe Asn His Ser Val Phe Asp Val Pro Leu 
        275                 280                 285             


His Tyr Gln Phe His Ala Ala Ser Thr Gln Gly Gly Gly Tyr Asp Met 
    290                 295                 300                 


Arg Lys Leu Leu Asn Gly Thr Val Val Ser Lys His Pro Leu Lys Ser 
305                 310                 315                 320 


Val Thr Phe Val Asp Asn His Asp Thr Gln Pro Gly Gln Ser Leu Glu 
                325                 330                 335     


Ser Thr Val Gln Thr Trp Phe Lys Pro Leu Ala Tyr Ala Phe Ile Leu 
            340                 345                 350         


Thr Arg Glu Ser Gly Tyr Pro Gln Val Phe Tyr Gly Asp Met Tyr Gly 
        355                 360                 365             


Thr Lys Gly Asp Ser Gln Arg Glu Ile Pro Ala Leu Lys His Lys Ile 
    370                 375                 380                 


Glu Pro Ile Leu Lys Ala Arg Lys Gln Tyr Ala Tyr Gly Ala Gln His 
385                 390                 395                 400 


Asp Tyr Phe Asp His His Asp Ile Val Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     


Ser Ser Val Ala Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ala Lys Arg Met Tyr Val Gly Arg Gln Asn Ala Gly Glu Thr 
        435                 440                 445             


Trp His Asp Ile Thr Gly Asn Arg Ser Glu Pro Val Val Ile Asn Ser 
    450                 455                 460                 


Glu Gly Trp Gly Glu Phe His Val Asn Gly Gly Ser Val Ser Ile Tyr 
465                 470                 475                 480 


Val Gln Arg 
            


<210> 5
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      peptide"

<400> 5
Thr Lys Gly Asp Ser Gln Arg Glu Ile 
1               5                   


<210> 6
<211> 6
<212> PRT
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      peptide"

<400> 6
Ile Pro Thr His Gly Val 
1               5       


